Crude and total fat determination in a feed sample by Soxhlet
This Application Note follows the official method EC No. 152/2009 from the Official Journal of the European Communities. The directive includes both direct extraction (crude fat) and extraction after acid hydrolysis (total fat). Which of the two procedures is used depends on the nature and composition of the sample. Both procedures are described in this Application Note. The directive EC No. 152/2009 requires an extraction time of 6 h. This Application Note shows by means of a certified reference material sample that an extraction time of 2 h is sufficient to receive reliable and reproducible results for crude fat as well as total fat using the FatExtractor E‑500 SOX.
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The FatExtractor E-500 is designed for quick and compliant fat extraction. Readily adapt your FatExtractor E-500 to changing needs with the interchangeable glass assembly and execute extractions according to Soxhlet, Randall or Twisselmann.
If you perform acid hydrolysis as a sample preparation step for total fat determination, discover HydrolEx H-506 as a complementary and robust addition to your FatExtractor E-500. Benefit from a smooth, safe process with convenient system handling.
Food Analysis Champion Guide Book
This guidebook offers a comprehensive summary of how NIR and reference methods, such as fat extraction and the Kjeldahl method for protein determination, are used at all steps in the food processing chain. Get a detailed overview of the techniques, including sample preparation, regulatory measures, troubleshooting, FAQs, and case-specific studies.