乳制品中的氮和蛋白质的测定(凯氏定氮片)
使用凯氏定氮片和过氧化氢测定乳制品中的氮和蛋白质
蛋白质测定是食品工业的一个关键分析项目。要求使用硫酸对样品进行消化吸收,以将氮转化为硫酸铵。过氧化氢用于加速消解过程。使用氢氧化钠通过碱化作用转化为氨后,利用蒸汽蒸馏法将样品蒸馏到硼酸接收器中,然后使用硫酸溶液进行滴定。将氮含量乘以样品特定蛋白质因子(乳制品为 6.38),得到蛋白质含量。
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Nitrogen and Protein Determination in Animal Feed according to the Kjeldahl method
The determination of protein in feed is a routine procedure for quality assurance and labelling. A simple and fast method for protein determination in feed products according to the ISO 5983-2:2009 and AOAC 954.01 regulations is introduced below. The sample is digested with sulfuric acid and Kjeldahl Tablets Titanium using the digest Automat K-438, followed by distillation and titration with the KjelMaster K-375 with KjelSampler K-376.