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Nitrogen and Protein Determination in Whey Protein Concentrates and Demineralized Whey Powder

Nitrogen and Protein Determination in Whey Protein Concentrates and Demineralized Whey Powder according to the Kjeldahl Method
Nitrogen and Protein Determination in Whey  Protein Concentrates and Demineralized Whey  Powder according to the Kjeldahl Method

Protein determination is one of the key analyses performed in the food industry. The samples require digestion with sulfuric acid to convert nitrogen into ammonium sulfate. After conversion to ammonia through the alkalinization with sodium hydroxide, the sample is distilled into a boric acid receiver by steam distillation, followed by a titration with sulfuric acid solution. The nitrogen content is multiplied by a sample-specific factor (6.38 for whey protein) to obtain the protein content.