Piperine determination in pepper using Universal­Extractor E-800

UniversalExtractor E-800: Extraction of piperine in pepper.

Piperine determination in pepper using UniversalExtractor E-800

Pepper is one of the most widely used spice and seasoning. Piperine – the bioactive compound of white, black or long pepper – imparts the spice with its pungency and biting taste. In this application note, the sample is extracted with the UniversalExtractor E-800 using the Hot extraction method. The total piperine content is determined using UV/Vis spectrophotometry. The procedure complies with the official method of AOAC 987.07. 

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