Flavonoid determination in calendula officinalis (marigold)

UniversalExtractor E-800: Extraction of marigold for the flavonoid determination.

A simple and reliable procedure for the extraction and determination of flavonoids in calendula officinalis (marigold) is introduced. The sample is extractd with the UniversalExtractor E-800 using the method Soxhlet warm. The flavonoid content is determined by using UV/Vis-spectrophotometry. This application complied with the official method of "Calendula flos" from the European Pharmacopoeia.

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