Separation of Vitamins
Separation of Vitamins
Vitamins B1 (thiamine) and B6 (pyridoxine) are essential for energy metabolism, nerve function, and immune health. Found in grains, legumes, meat, and bananas, their similar structures make separation challenging. Preparative high pressure liquid chromatography (prep HPLC) offers high-resolution purification, ensuring pure compounds for precise pharmaceutical and nutritional applications. The following application demonstrates the use of prep HPLC to efficiently isolate B1 and B6 from a vitamin mixture.