Applications: Lyophilisateurs

Determination of Polycyclic Aromatic Hydrocarbons in water using SyncorePlus with Solid Phase Extraction (SPE) cover

SyncorePlus Analyst:  Determination of Polycyclic Aromatic Hydrocarbons in water using SyncorePlus with Solid Phase Extraction (SPE) cover

Endpoint of freeze drying by pressure difference test

The pressure difference test in manifold drying chamber on Lyovapor™ is used to determine the end of the primary drying phase. A range of water-solvent mixtures is analyzed to identify conditions enabling reliable implementation for this test.

Extraction of micropollutants from contaminated sediments after heavy rain events

Pressurized solvent extraction (PSE) of contamined sediments as a critical part of aquatic risk accessment

L'utilisation de solvants organiques dans les lyophilisateurs de laboratoire

Freeze-dryers were initially intended to be used with water as a solvent. With new applications, freeze-drying is consequently being used more and more in combination with organic solvents instead of water. Organic solvents and inorganic acids and bases are often used in research and development prior to lyophilisation of the sample. The behavior of these new types of samples during freeze-drying must therefore be considered prior to the process.

Freeze-Drying of deepwell plates using Frontloader drying chamber

Deepwell plates, also microwell plate, are often found in pharmaceutical and biological laboratories for processing a large number of samples. In this application note, BUCHI Frontloader drying chamber demonstrates the successful freeze drying of 300 uL deepwell plates using a Lyovapor™ system. combined with the The high number of samples can be freeze dried in one plate allows quick  and easy handling. This includes freeze-drying as well. T

Freeze-Drying of banana sample in Frontloader drying chamber

Fruits such as bananas, with their delicate texture and high moisture content, present unique challenges in preservation. Anyhow, the sample throughput is limited by available surface in freeze dryer since only a monolayers of sample can be dried in parallel. This application note highlights the process parameters, advantages, and outcomes of banana freeze-drying in a Frontloader drying chamber, offering insights into improved stability and storage.  

Contamination-free and sterile freeze-drying using lyophilization bag

Freeze-drying is typically used to deal with sensitive and expensive samples as it offers a gentle drying process. One way to avoid contamination in the drying process is the use of a container with membrane that protect samples from contamination and secondly keep product flyout in place which protects multiple samples from cross contamination.  

Preserving peptide integrity by freeze-drying

Following purification by HPLC, peptide solutions are typically freeze‑dried to remove solvents, a process that preserves structural integrity and biological activity while minimizing aggregation. Samples are processed in beaker flasks to facilitate easy collection and reduce product loss. The resulting lyophilized material is consistently obtained as a fine, fluffy powder without collapse or stickiness, ensuring physical stability suitable for long‑term storage and downstream therapeutic or research applications

Stabilizing oligonucleotides for long-term storage by freeze-drying

Freeze-drying preserves their integrity and extends shelf life of Oligonucleotides, short DNA or RNA sequences. Using the Lyovapor™ L-250, 50 mL Falcon tube samples were rapidly frozen in liquid nitrogen and dried in a 12-port manifold at 0.1 mbar, producing stable pellets in 16 hours. Multiple trials showed reproducible results, demonstrating reliable vacuum control, and effective vapor trapping. 

Freeze-dried Okra Vegetable

Okra (Abelmoschus esculentus), due to its high moisture content and sensitive structure, is difficult to dehydrate using conventional drying techniques. This application note evaluates the freeze-drying of okra using the Lyovapor™ L-210 freeze-dryer. The low-temperature, low-pressure drying process enables efficient moisture removal by sublimation, resulting in dried okra with low residual moisture and well-preserved structure and appearance. The high-quality freeze-dried okra can stored safely and used for functional food applications

Freeze drying of Pharmaceutical Ingredients

BUCHI Lyovapor™ L-250 Basic is applied in freeze drying active pharmaceutical compounds dissolved in organic solvent mixtures.

Polycyclic aromatic hydrocarbons in sludge samples

Lyovapor™ L-210 Pro, SpeedExtractor E-916, Syncore Analyst: Pressurized Solvent Extraction of a sludge sample for the determination of polycyclic aromatic hydrocarbons (PAHs)

Lyophilisation of truffles

Lyophilisation of apples

Lyovapor™ L-210: Freeze Drying of apples We developed a freeze-drying method with optimized process time and residual moisture content for apples.

Lyophilisation of strawberries

Lyovapor™ L-200: Freeze Drying of strawberries We developed a freeze-drying method with optimized process time and residual moisture content for strawberries.

Lyophilisation_de_la_tige_de_Giloy.pdf

Dans la médecine indienne traditionnelle, le guduchi est l'une des plantes ayurvédiques les plus utiles. La lyophilisation est évaluée comme méthode de séchage afin de préserver les propriétés du guduchi. La lyophilisation est reconnue pour être l'une des méthodes les plus appropriées pour la préservation des matières naturelles et biologiques grâce à l'environnement dépourvu d'oxygène et aux faibles températures de fonctionnement.

Lyophilisation of fresh banana slices