Sepiatec SFC Systems

With Sepiatec SFC systems, fractions are collected in an unpressurized state. Thereby, not only is safety increased but also handling is simplified. There is no limitation concerning the size of fraction volumes. This option proves to be highly convenient when working with stacked injections.

Monitoring the separation of a mixture's compounds is crucial for successful purification as it ensures the proper collection of the target compound. All Sepiatec SFC systems are equipped with a UV detector but also allow the connection of an Evaporative Light Scattering Detector (ELSD) and Mass Spectrometer (MS). Sample injection can be done on the Sepiatec SFC instrument in two ways: multiple and stacked. Stacked injections are useful when injecting large sample amounts under isocratic solvent conditions, while multiple injections are done sequentially.

Sepiatec SFC instrument design stands out due to its modular design, which is highly service-friendly by allowing access to all parts from the front. Replacement of spare or worn parts is quick and easy as there is no need to move or turn the instrument.

Even being the smallest on the market, Sepiatec SFC systems come with everything required to run supercritical fluid chromatography without compromise.

Stack injection mode is used when working with large sample amounts and requires an isocratic gradient mode. This technique allows for the successive injection of additional samples and utilizes the time spent waiting for peak elution. The Sepiatec Prep SFC Control software automatically suggests stacking time and therefore makes the setup of a stacked injection method extraordinarily simple.

The entire Sepiatec SFC device runs dedicated software developed by SFC experts. A clear menu structure enables a simple and intuitive process with dedicated functions such as stack injection, peak detection, and on-the-fly editing for changing parameters during a run. All input options are presented on just a few screens. Data is entered via the 15.6‘‘ touchscreen, which has been placed ergonomically on the upper front of all Sepiatec SFC systems.

All Sepiatec SFC instruments, from small to large-scale purification, require a minimum footprint in the laboratory. The system controller and fraction collector are built into the device and need no extra space beside the instrument. Therefore, precious lab space, be it in or out of a fume hood, can be saved and used for other equipment.

Sepiatec SFC systems cover the full range of separation needs, handling sample capabilities in the mg to gram range. Pumps with different flow rate ranges allow for prep HPLC columns of various sizes, accommodating internal diameters ranging from 4-16mm, 15-30mm, and 30-50mm and lengths from 150-800mm. Depending on the model, up to 10 columns can be stored in the column oven simultaneously and connected for screening if required.

A simple setup makes becoming familiar with the instrument a breeze; therefore, time-intensive training sessions are unnecessary. Everything has been thought of, including a connection piece for an exhaust system by default. In addition, all Sepiatec SFC systems can be operated on a benchtop or a fume hood.