Nitrogen determination in diphtheria and tetanus toxoids according to Pharmacopoeia using back titration and boric acid titration
KjelMaster System K-375 / K-376, KjelDigester K-449, Scrubber K-415
Nitrogen and total protein determination in pharmaceutical products are described in the European Pharmacopoeia (Ph. Eur.) and in the Pharmacopoeia of the United States (USP). Therein, different titration techniques are specified. The Ph.Eur. is using back titration in the presence of the indicator methyl red mixed solution and the USP is using potentiometric and colorimetric boric acid titration.In this application note, the three different titration techniques were compared and the reproducibility was determined. The nitrogen content of two toxoid samples was determined by sulfuric acid digestion using the KjelDigester K-449 followed by steam distillation and titration applying the BUCHI KjelMaster System K-375 / K-376.
Register for download
Related Instruments
KjelMaster K-375 & KjelSampler K-376 / K-377
Perform potentiometric and colorimetric titrations with the KjelMaster K-375 and a free choice of methods at perfect usability. Gain high throughput, more automation and sophisticated data management for convenient nitrogen and protein analysis.
Scrubber K-415
With four steps to occupational safety, Scrubber K-415 provides you with maximal protection. The unit neutralizes poisonous fumes and reaction gases and minimizes use of cooling water to safeguard both you and the environment.
KjelDigester K-446 / K-449
The KjelDigester K-446 / K-449 reduces the block digestion process by up to 2 hours and meets the highest safety requirements. The high degree of automation is the perfect match to the KjelMaster System allowing more samples per day to be processed.
