Preserving peptide integrity by freeze-drying

Contamination-free and sterile freeze-drying using lyophilization bag

Freeze-drying is typically used to deal with sensitive and expensive samples as it offers a gentle drying process. One way to avoid contamination in the drying process is the use of a container with membrane that protect samples from contamination and secondly keep product flyout in place which protects multiple samples from cross contamination. 

Endpoint of freeze drying by pressure difference test

The pressure difference test in manifold drying chamber on Lyovapor™ is used to determine the end of the primary drying phase. A range of water-solvent mixtures is analyzed to identify conditions enabling reliable implementation for this test.

Freeze drying of Pharmaceutical Ingredients

BUCHI Lyovapor™ L-250 Basic is applied in freeze drying active pharmaceutical compounds dissolved in organic solvent mixtures.

Stabilizing oligonucleotides for long-term storage by freeze-drying

Freeze-drying preserves their integrity and extends shelf life of Oligonucleotides, short DNA or RNA sequences. Using the Lyovapor™ L-250, 50 mL Falcon tube samples were rapidly frozen in liquid nitrogen and dried in a 12-port manifold at 0.1 mbar, producing stable pellets in 16 hours. Multiple trials showed reproducible results, demonstrating reliable vacuum control, and effective vapor trapping. 

The use of organic solvents in laboratory freeze-dryer

Freeze-dryers were initially intended to be used with water as a solvent. With new applications, freeze-drying is consequently being used more and more in combination with organic solvents instead of water. Organic solvents and inorganic acids and bases are often used in research and development prior to lyophilisation of the sample. The behavior of these new types of samples during freeze-drying must therefore be considered prior to the process.

Freeze-Drying of deepwell plates using Frontloader drying chamber

Deepwell plates, also microwell plate, are often found in pharmaceutical and biological laboratories for processing a large number of samples. In this application note, BUCHI Frontloader drying chamber demonstrates the successful freeze drying of 300 uL deepwell plates using a Lyovapor™ system.

combined with the The high number of samples can be freeze dried in one plate allows quick 

and easy handling. This includes freeze-drying as well. T