Stabilizing oligonucleotides for long-term storage by freeze-drying

Contamination-free and sterile freeze-drying using lyophilization bag

Freeze-drying is typically used to deal with sensitive and expensive samples as it offers a gentle drying process. One way to avoid contamination in the drying process is the use of a container with membrane that protect samples from contamination and secondly keep product flyout in place which protects multiple samples from cross contamination. 

Endpoint of freeze drying by pressure difference test

The pressure difference test in manifold drying chamber on Lyovapor™ is used to determine the end of the primary drying phase. A range of water-solvent mixtures is analyzed to identify conditions enabling reliable implementation for this test.

Freeze drying of Pharmaceutical Ingredients

BUCHI Lyovapor™ L-250 Basic is applied in freeze drying active pharmaceutical compounds dissolved in organic solvent mixtures.

Preserving peptide integrity by freeze-drying

Following purification by HPLC, peptide solutions are typically freeze‑dried to remove solvents, a process that preserves structural integrity and biological activity while minimizing aggregation. Samples are processed in beaker flasks to facilitate easy collection and reduce product loss. The resulting lyophilized material is consistently obtained as a fine, fluffy powder without collapse or stickiness, ensuring physical stability suitable for long‑term storage and downstream therapeutic or research applications

The use of organic solvents in laboratory freeze-dryer

Freeze-dryers were initially intended to be used with water as a solvent. With new applications, freeze-drying is consequently being used more and more in combination with organic solvents instead of water. Organic solvents and inorganic acids and bases are often used in research and development prior to lyophilisation of the sample. The behavior of these new types of samples during freeze-drying must therefore be considered prior to the process.

Freeze-Drying of deepwell plates using Frontloader drying chamber

Deepwell plates, also microwell plate, are often found in pharmaceutical and biological laboratories for processing a large number of samples. In this application note, BUCHI Frontloader drying chamber demonstrates the successful freeze drying of 300 uL deepwell plates using a Lyovapor™ system.

combined with the The high number of samples can be freeze dried in one plate allows quick 

and easy handling. This includes freeze-drying as well. T