Sample concentration Amifostine/PLGA (0.4:1.0 and 1:1), Amifostine (400mg/1ml or 1000 mg/2ml) in de-ionized water and emulsified in 50mL of dichloromethane containing 1 g of PLGA Solvent Amifostine in Water, PLGA in DCM Carrier, Filmogen Poly (DL-lactic–glycolic acid) PLGA 50:50 (RG 502; inherent viscosity 0.2 dL/g), Phosphatidylcholine (Surfactant)