Why a 100 bar purification system?
For most purification applications, decreasing the silica particle size down to 7 or 10 µm allows obtaining the required resolution in order to collect a pure compound. Further decreasing it to 5 µm is usually not necessary and would increase substantially the price of the column:
Which prep HPLC columns can be used?
Purifications can be performed using prep HPLC columns with a diameter up to 80 mm filled with silica having a particle size down to 7 µm.
What is the standard flow rate and sample load of a prep HPLC column?
The following table gives general indications. The indicated values vary according to the type of sample, the required resolution, the length of the column, and the operating conditions.
How many solvents can be handled with PrepChrom C-700?
The pump can handle 4 solvents for a true ternary/quaternary gradient. An adjustable mixing chamber improves gradient accuracy.
Which is the maximum sample amount that can be purified with the PrepChrom C-700?
The system works with flash columns up to 800g and prep HPLC columns with a diameter of 50mm. This allows a maximum sample loading capacity of 80g for flash chromatography and 1g for prep HPLC depending on the type of sample and column.
Which wavelengths range can be chosen for UV detection?
The integrated UV detector provides four wavelengths and a scan function in the range 200-600nm.
Which detectors can be externally connected to PrepChrom C-700?
The BUCHI C-650 ELS detector can be easily controlled. In addition, a second detector can be optionally connected through an analog signal input.
Does PrepChrom C-700 feature the possibility to generate automatically a gradient from TLC data?
Yes, the system utilizes the Rf values from TLC and automatically calculates the optimal gradient conditions.
Can the UV scan also be observed after the run?
The UV scan can be observed in real-time or analyzed after the run.
How is the injection of the sample managed?
The PrepChrom C-700 is equipped with an automated 6-port injection valve. This valve can be either connected with a loop (liquid injection) or with a pre-column (solid injection).