A procedure for the oil determination in a soybeans sample following the official method AOCS Am 2-93 Oil Content in Oilseeds by the American Oil Chemists` Society is introduced. The standard method Am 2-93 is equal to the International Standard ISO 659:2009 and the FOSFA International Official Method, Oilseeds – Determination of oil content – solvent extraction (reference method). The standard methods require an extraction with a Butt-type extraction apparatus. The Butt-type extraction is equal to the Twisselmann extraction method used in the FatExtractor E-500 ECE.
Both, the Butt-type extractor as well as the Economic Continuous Extraction (ECE) are continuous extractions where the sample is constantly kept in hot solvent vapor whilst efficiently rinsed with freshly distilled solvent. The oil content is determined gravimetrically after the extract has been dried to a constant weight.
AOCS Am 2-93, which is equal to ISO 659, describes a determination consisting of three extractions: the sample is first extracted for 4 h, then the sample is grinded, followed by a second extraction for 2 h, the sample is grinded again and extracted with a third extraction for 2 h.
This Application Note shows by means of a certified reference material sample that reliable and reproducible results will be received using the FatExtractor E-500 ECE.