Determination of flavonoid content in calendula officinalis (marigold)

Extraction of marigold for the flavonoid determination using the UniversalExtractor E-800
Determination of flavonoid content in calendula officinalis (marigold)

A simple and reliable procedure for determination of flavonoids content in calendula officinalis (marigold) is introduced. Flavonoids are polyphenolic secondary metabolites with a low bioavailability for the human body. Once absorbed, the flavonoids are rapidly metabolized, resulting in metabolites with anti-inflammatory, -oxidant, -thrombogenic, -diabetic and -cancer properties. Flavonoids are found in many plants and plant-based products that are commonly consumed, such as fruits, vegetables, wine or tea. One example represents the calendula officinalis, also known as marigold, which is described in this application note. Due to the low bioavailability of flavonoids, in combination to the rapid metabolization in the human body, even high intake of flavonoids is regarded to be safe.
In the presented application, the sample is extracted with the UniversalExtractor E-800 using the method Soxhlet warm. The flavonoid content is determined by using UV/Vis-spectrophotometry. This application complies with the official method of ‘Calendulae flos’ from the European Pharmacopoeia.