응용자료

Determination of oil contents in seed samples

Bioactive oils are commonly used in food, pharmaceutical and cosmetic applications. Those oils are usually rich in unsaturated and polyinsaturated fatty acids. The majority of fatty acids can be produced by the human body. However, omega-3 and omega-6, essential in human nutrition, cannot be synthesized and must be provided by food. Therefore, oils rich in unsaturatted fatty acids are often used in food preparations or for direct consumption. In this study, two premium local oils representing a high linoleic (hemp) and a high linolenic (linseed) acid content were chosen, together with a novel high linoleic acid containing oil extracted from chia seeds. The objective of this work was to extract and to quantify the oil content from these seeds with a Pressurized Solvent Extraction (PSE) method using the SpeedExtractor E-916 and to compare the results with classical extraction methods. Therefore, the samples were extracted with a Soxhlet extraction method using the Extraction Unit E-816 SOX for the determination of crude fat and with a Weibull Stoldt method using a Hydrolysis Unit E-416 followed by a Soxhlet extraction method using the Extraction Unit E-816 SOX for the determination of total fat. The fat content is determined gravimetrically after the extract has been dried to a constant weight.

Ethyl carbamate in alcoholic beverages

Port wine samples were analyzed for its ethyl carbamate (urethane) content by the Instituto dos Vinhos do Douro e do Porto, I.P. (IVDP) according to the method OIV-MA-AS 315-04. Parallel solid phase extraction and parallel evaporation was performed by employing a Syncore® Analyst equipped with an SPE Advanced cover. 14 wine samples were analyzed to validate the method yielding a mean recovery rate of 99.1 % for ethyl carbamate with a standard deviation of 12.5 %.

Extraction of Soil using the SpeedExtractor E-916 for the Determination of Pesticides

A pesticide is any substance or mixture of substances intended for, preventing, destroying, repelling, or mitigating any pest. There are three main types of pesticides: organochlorine, organophosphate and carbamates. OCP can cause severe health problems, are persistent and tend to bio-accumulate. Therefore the use of some OCP is banned by the Stockholm convention

Extraction and concentration of turmeric using a Rotavapor®

Fat determination in Yoghurt and Curt Cheese using the SpeedExtractor E-916

Pressurized Liquid Extraction (PLE) is a well-established alternative for extracting lipids and/or various components faster and cheaper, but less frequently used for the verification of fat labels in food products. A reliable and fast procedure using PLE to determine the fat content in samples with easily accessible fat and high moisture content (above 50 %) is presented below. The extraction was carried out using the SpeedExtractor E-916 in combination with Multivapor™ P-12 for solvent evaporation in parallel. The fat content was determined gravimetrically.

Fat in Polymer

A quick and easy method for the determination of the fat content in polymer samples is introduced. The samples are extracted simultaneously under high pressure and elevated temperature with the SpeedExtractor E-916 using a pressurized solvent extraction (PSE)

Fat Determination in Shortbread using the SpeedExtractor E-916

The determination of fat in food and feed is a routine procedure for in quality assurance and labeling. Fat was determined in butter shortbread after homogenization of the sample and extraction with the SpeedExtractor E-916. The solvent was evaporated in parallel using the Multivapor P-6. The total fat content was determined gravimetrically after the extract has been dried to a constant weight.

Fat determination in food and feed products using SpeedExtractor E-914 after Acid Hydrolysis

Fat determination is one of the key analyses performed in the food industry. Complex matrices such as food typically require acid hydrolysis prior to solvent extraction, to free bound lipids and to facilitate the extraction. An easy and reliable procedure to determine the fat content in different food and feed matrices is presented below.

Fat determination in Mayonnaise and Chocolate using the SpeedExtractor E-916

 Pressurized Liquid Extraction (PLE) is a well-established alternative for efficient and cost-effective extraction of lipids and various components. However, its application for verifying fat labels in food products is less common. In this context, a reliable and rapid PLE procedure is presented for determining the fat content in samples with easily accessible fat, particularly in mayonnaise and chocolate. For the extraction, the renowned Buchi Product, the SpeedExtractor E-916, was employed in combination with the Multivapor™ P-12 for parallel solvent evaporation. This powerful combination enabled swift and efficient extraction of lipids from the samples. Subsequently, the fat content was determined gravimetrically after the extract had been dried to a constant weight.The application of PLE, utilizing Buchi Products, in the determination of fat content in mayonnaise and chocolate offers significant advantages. It ensures precise quantification, saving time and cost compared to conventional methods. By employing PLE with the SpeedExtractor E-916 and Multivapor™ P-12, manufacturers, quality control laboratories, and regulatory bodies can reliably verify fat labels in these popular food products, contributing to accurate labeling and consumer satisfaction.  

Determination of polycyclic aromatic hydrocarbons in water

Metabolites of polycyclic aromatic hydrocarbons (PAH) have been shown to act as potent carcinogens, mutagens and teratogens. Thus, the determination of PAH is of great importance. A simple and fast method for their determination in waste water is introduced below. The sample is eluted with dichloromethane (DCM) directly into the Syncore® Analyst sample tube after being extracted over a solid phase extraction (SPE) cartridge. Subsequently, the DCM is evaporated to a residual volume for GC-MS analysis. The combined extraction-evaporation sample preparation method proceeds without cross-contamination.

GIN: traditional production versus modern techniques

Gin is an old medicine as well as a trendy liquor. The Juniper flavored spirit has been in existence for hundreds of years. In recent years gin has experienced a renaissance. It has become one of the trendiest spirits. We find the traditional famous gins on the market as well as gins with completely different flavor profiles and distillation techniques.

Improved analyte recovery with keeper solvents

Polychlorinated biphenyls are suspected endocrine disruptors. They are enriched in the food chain which can result in elevated concentrations in fish and meat. The concentration of some PCBs is the subject of regulations and must be analyzed by reliable methods. Often, PCBs are extracted from a matrix. The obtained extract must be concentrated before analysis. It is then crucial to maintain a high analyte recovery in the concentration procedure.

Labor free, eco-friendly concentrations – comply with US EPA regulations

Environmental waste, soil, water and air samples are usually concentrated before analysis. During the concentration step, traditionally performed by Kuderna-Danish (KD) or nitrogen blowdown devices, organic solvent fumes could escape to the environment. Thus, condensation of solvent vapors is inefficient, if possible at all. These solvent vapors are harmful to exposed operators and persist in the atmosphere where they may accelerate global warming. Obviously, environmental laboratories play a pioneering role in benign use and recycling of organic solvents. Therefore, environmental laboratories are strictly controlled. Special attention is paid on chlorinated solvent emission monitoring. Methylene chloride, for example, has a high global warming potential and is considered a probable human carcinogen. High solvent emissions and noncompliance with US EPA (United States Environmental Protection Agency) regulations could lead to severe monetary fines. We present the Syncore® technology applied in automated parallel concentration of samples and recuperation of organic solvents. Selected US EPA applications are listed where the Syncore® Analyst has been proven to be a “working horse” for the environmental chemist.

Fat determination in Food and Feed Products using SpeedExtractor E-916 after Acid Hydrolysis

Fat determination is one of the key analyses performed in the food industry. Complex matrices such as food typically require acid hydrolysis prior to solvent extraction, to free bound lipids and to facilitate the extraction. An easy and reliable procedure to determine the fat content in different food and feed matrices is presented below.

Concentration and solvent recovery of environmental samples in dichloromethane

Environmental samples are usually extracted with an organic solvent which is then concentrated prior to final analysis.In this Short Note, initial conditions and evaporation settings for the parallel concentration of samples dissolved in dichloromethane (DCM) having different starting volumes are presented. These settings have been optimized for high analyte and high solvent recoveries.

Determination of hexachlorocyclohexane isomers and chloro substituted benzene congeners in air

In the past, different hexachlorocyclohexane isomers and chloro substituted benzenes were produced and industrially applied in large quantities. Many of these substances have been deposited, accumulated and are still released in the environment from various sources today. The contaminants can be routed back to incomplete combustion, leaching of landfill sites or inadequate production of chlorinated compounds or pesticides. Analytical contract laboratories, usually concentrate environmental samples from many different matrices prior to the analysis by GC-MS. mas I münster analytical solutions GmbH, established methods for the determination of these contaminants from environmental screenings.In this short note, the use of the Syncore; as a time saving and reliable instrument for the determination of HCH and chloro-substituted benzenes in ambient air is illustrated.

Determination of Polymer Plasticizers in Polyamide samples using the SpeedExtractor E-916

The optimal properties of polyamides depend on the content of polymer plasticizers. This application note describes the extraction and the subsequent gravimetrical determination of polymer plasticizers in a polyamide PA 6 and a polyamide PA 12 sample using the SpeedExtractor E-916.

Determination of Hydrocarbon Oil Index in Water According to ISO 9377-2, Sample Clean-up and Concentration

Process water, surface water, drinking water, waste water and treated waste water are routinely tested for their amount of oil and grease – a pivotal water quality parameter. The oil and grease content in water is determined as a sum parameter for nonpolar C10 to C40 hydrocarbons. Details of the procedure are described in the ISO 9377-2 method. In this method, usually named H53, the hydrocarbon content is determined by a GC-FID.

Dioxin determination in ambient air

The Soxhlet extraction of dioxins from different environmental matrices is described by the regulated methods EPA 1613. This Application note describes the extraction of dioxins from particulate matter air samples collected on quartz fiber filters and on polyurethane foam cartridges using the BUCHI UniversalExtractor E-800. The extracts were analyzed by GC-MS.

Determination of Polycyclic Aromatic Hydrocarbons in water using SyncorePlus with Solid Phase Extraction (SPE) cover

PAHs are molecules based on aromatic rings containing only carbon and hydrogen atoms. PAHs are natural components of fossil fuels; they are released to the environment during incomplete combustion of organic matter. A spiked water sample is extracted using a Solid Phase Extraction (SPE) cartridge on the SyncorePlus SPE cover.