In the dairy industry, the fat determination and extraction is not only a routine procedure in quality assurance and labelling also it is very important for contaminant determination, as e.g. PCBs, dioxins or pesticides.
A procedure for fat determination in milk powder and infant formula samples is introduced. The samples are extracted by applying elevated pressure and temperature with the SpeedExtractor E-916. After drying the extract to a constant weight, the total fat content is determined gravimetrically.
The samples are not hydrolyzed prior to the extraction - hence, the process time is reduced and chemicals saved. To validate this approach, fat determination was performed with a prior hydrolysis step.