Stabilizing Oligonucleotides by freeze-drying
Oligonucleotide solutions containing a mixture of 20% acetonitrile and water were lyophilizeed to preserves their integrity and extends shelf life using the Lyovapor™ L-250 Basic.
Oligonucleotide samples in 50 mL Falcon tube samples were rapidly frozen in liquid nitrogen and dried in a 12-port manifold at 0.1 mbar, producing stable pellets in 16 hours. Multiple trials showed
reproducible results, demonstrating reliable vacuum control, and effective vapor trapping.