A simple and reliable procedure for determination of fat content in different food samples according to Weibull-Stoldt, Hot Extraction and Twisselmann Extraction is introduced. The samples are hydrolyzed using the HydrolEx H-506. The extraction is performed with the UniversalExtractor E‑800. Gravimetric determination of the total fat content follows the drying of the extract to a constant weight. This application complies with official methods (EN 98/64/EG, AOAC 963.15, ISO 22630:2015, AOAC 991.36). The combination of the HydrolEx H-506 and the UniversalExtractor E-800 increases the sample throughput.